製品の概要

  • 製品名Anti-SLC25A20 antibody
    SLC25A20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SLC25A20
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 215-264 (GGIAGIFNWA VAIPPDVLKS RFQTAPPGKY PNGFRDVLRE LIRDEGVTSL) of Human SLC25A20 (NP_000378).

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

ターゲット情報

  • 関連性SLC25A20 is one of several closely related mitochondrial membrane carrier proteins that shuttle substrates between cytosol and the intramitochondrial matrix space. It mediates the transport of acylcarnitines into the mitochondrial matrix for their oxidation by the mitochondrial fatty acid oxidation pathway. Mutations in this gene are associated with carnitine acylcarnitine translocase deficiency, which can cause a variety of pathological conditions such as hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and is usually lethal in new born and infants.
  • 細胞内局在Mitochondrion inner membrane; Multipass membrane protein.
  • 参照データベース
  • 別名
    • CAC antibody
    • CACT antibody
    • Carnitine/acylcarnitine translocase antibody
    • MCAT_HUMAN antibody
    • Mitochondrial carnitine/acylcarnitine carrier protein antibody
    • Solute carrier family 25 (carnitine/acylcarnitine translocase) member 20 antibody
    • Solute carrier family 25 member 20 antibody
    see all

Anti-SLC25A20 antibody 画像

  • Anti-SLC25A20 antibody (ab82678) at 1 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-SLC25A20 antibody (ab82678) 使用論文

This product has been referenced in:
  • Soni MS  et al. Downregulation of carnitine acyl-carnitine translocase by miRNAs 132 and 212 amplifies glucose-stimulated insulin secretion. Diabetes 63:3805-14 (2014). WB ; Rat . Read more (PubMed: 24969106) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375)
Loading amount 20 µg
Specification A375
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"