Anti-SLC25A13/Citrin 抗体 (ab96303)
Key features and details
- Rabbit polyclonal to SLC25A13/Citrin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SLC25A13/Citrin antibody
SLC25A13/Citrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLC25A13/Citrin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Zebrafish -
免疫原
Recombinant fragment corresponding to Human SLC25A13/Citrin aa 248-505.
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ポジティブ・コントロール
- IHC-P: Mouse kidney tissue;N87 xenograft tissue. ICC/IF: HeLa cells. WB: Mouse liver lysate; rat liver lysate; HepG2, HEK-293T, A431 and HeLa whole cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96303の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
ターゲット情報
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機能
Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. -
組織特異性
High levels in liver and low levels in kidney, pancreas, placenta, heart and brain. -
関連疾患
Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2) [MIM:603471]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years.
Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD) [MIM:605814]. NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms. -
配列類似性
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 615470 Cow
- Entrez Gene: 10165 Human
- Entrez Gene: 50799 Mouse
- Entrez Gene: 362322 Rat
- Omim: 603859 Human
- SwissProt: Q9UJS0 Human
- SwissProt: Q9QXX4 Mouse
- Unigene: 489190 Human
see all -
別名
- AI785475 antibody
- ARALAR2 antibody
- Calcium binding mitochondrial carrier protein Aralar2 antibody
see all
画像
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SLC25A13/Citrin using ab96303 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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All lanes : Anti-SLC25A13/Citrin antibody (ab96303) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa -
Paraffin-embedded mouse kidney tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: Citrate buffer, pH 6.0, 15 min.
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Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + Mouse liver lysate at 50 µg
Predicted band size: 74 kDa -
Paraffin-embedded N87 xenograft tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EWDTA-based (pH 8.0), 15 minutes.
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Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 74 kDa -
Anti-SLC25A13/Citrin antibody (ab96303) at 1/500 dilution + Rat liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96303 は 1 報の論文で使用されています。
- Koshenov Z et al. Citrin mediated metabolic rewiring in response to altered basal subcellular Ca2+ homeostasis. Commun Biol 5:76 (2022). PubMed: 35058562