製品の概要

  • 製品名Anti-SLC25A12 antibody
    SLC25A12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SLC25A12
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide, corresponding to a region within internal sequence amino acids 612-661 (LKPAGSEPTP KSRIADLPPA NPDHIGGYRL ATATFAGIEN KFGLYLPKFK) of Human SLC25A12, (NP_003696)

  • ポジティブ・コントロール
    • COLO205 cell lysate

アプリケーション

Our Abpromise guarantee covers the use of ab90095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle.
  • 組織特異性High levels in heart and skeletal muscle, low in brain and very low in kidney.
  • 関連疾患Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system.
  • 配列類似性Belongs to the mitochondrial carrier family.
    Contains 4 EF-hand domains.
    Contains 3 Solcar repeats.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGC1 antibody
    • Araceli hiperlarga antibody
    • ARALAR antibody
    • ARALAR1 antibody
    • Calcium binding mitochondrial carrier superfamily member antibody
    • Calcium binding mitochondrial carrier superfamily member Aralar1 antibody
    • Calcium-binding mitochondrial carrier protein Aralar1 antibody
    • CMC1_HUMAN antibody
    • Mitochondrial aspartate glutamate carrier 1 antibody
    • SLC25A12 antibody
    • Solute carrier family 25 (aspartate/glutamate carrier) member 12 antibody
    • Solute carrier family 25 (mitochondrial carrier Aralar) member 12 antibody
    • Solute carrier family 25 member 12 antibody
    • solute carrier family 25, member 12 antibody
    see all

Anti-SLC25A12 antibody 画像

  • Anti-SLC25A12 antibody (ab90095) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa

Anti-SLC25A12 antibody (ab90095) 使用論文

This product has been referenced in:
  • Povlsen JA  et al. Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation. PLoS One 8:e64093 (2013). WB ; Rat . Read more (PubMed: 23704975) »
  • Zhong Q & Kowluru RA Diabetic retinopathy and damage to mitochondrial structure and transport machinery. Invest Ophthalmol Vis Sci 52:8739-46 (2011). WB ; Rat . Read more (PubMed: 22003103) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375 cell line)
Loading amount 20 µg
Specification A375 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"