製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC24A6
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: SIGDAFSDFT LARQGYPRMA FSACFGGIIF NILVGVGLGC LLQISRSHTE , corresponding to a region within internal sequence amino acids 468 - 517 of Human SLC24A6 (NP_079235)

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa.Can be blocked with SLC24A6 peptide (ab201524). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Transports Ca(2+) in exchange for either Li(+) or Na(+), explaining how Li(+) catalyzes Ca(2+) exchange. In contrast to other members of the family its function is independent of K(+).
  • 配列類似性
    Belongs to the sodium/potassium/calcium exchanger family. SLC24A subfamily.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ22233 antibody
    • Na(+)/K(+)/Ca(2+) exchange protein 6 antibody
    • Na(+)/K(+)/Ca(2+)-exchange protein 6 antibody
    • NCKX6 antibody
    • NCKX6_HUMAN antibody
    • NCLX antibody
    • Slc24a6 antibody
    • Sodium/potassium/calcium exchanger antibody
    • Sodium/potassium/calcium exchanger 6 antibody
    • Solute carrier family 24 (sodium/potassium/calcium exchanger) member 6 antibody
    • Solute carrier family 24 member 6 antibody
    see all

画像

  • Anti-SLC24A6 antibody (ab83551) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 35 kDa,95 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab83551 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Guinea pig Cell lysate - other (heart mitochondria)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
heart mitochondria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ting

Verified customer

投稿 Aug 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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