The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Critical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca(2+) and 1 K(+) in exchange for 4 Na(+).
組織特異性Expressed in the retina, particularly in the inner segment, outer and inner nuclear layers, and ganglion cell layer.
関連疾患Defects in SLC24A1 are the cause of congenital stationary night blindness type 1D (CSNB1D) [MIM:613830]. An autosomal recessive form of congenital stationary night blindness a non-progressive retinal disorder characterized by impaired night vision. CSNB1D is characterized by a Riggs type of electroretinogram (proportionally reduced a- and b-waves). Patients have visual acuity within the normal range and no symptoms of myopia and/or nystagmus.
配列類似性Belongs to the sodium/potassium/calcium exchanger family. SLC24A subfamily.
翻訳後修飾The uncleaved signal sequence is required for efficient membrane targeting and proper membrane integration.