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Recombinant fragment corresponding to amino acids 451-550 of Human SLC22A6 with proprietary 26 kDa tag (AAH33682).
Our Abpromise guarantee covers the use of ab118346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23516613|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 62 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.|
|IHC-P||Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Sandwich ELISA||Use a concentration of 10 µg/ml.|
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