製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to SLC22A6
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-P, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 451-550 of Human SLC22A6 with proprietary 26 kDa tag (AAH33682).

  • ポジティブ・コントロール
    • Human kidney tissue
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23516613
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 62 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
  • 組織特異性
    Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.
  • 配列類似性
    Belongs to the major facilitator superfamily. Organic cation transporter family.
  • ドメイン
    Multiple cysteine residues are necessary for proper targeting to the plasma membrane.
  • 翻訳後修飾
    Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necesssary for proper targeting of the transporter to the plasma membrane.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ55736 antibody
    • hOAT1 antibody
    • hPAHT antibody
    • hROAT1 antibody
    • MGC45260 antibody
    • OAT1 antibody
    • Organic anion transporter 1 antibody
    • OTTHUMP00000236796 antibody
    • OTTHUMP00000236797 antibody
    • OTTHUMP00000236798 antibody
    • OTTHUMP00000236799 antibody
    • PAH transporter antibody
    • PAHT antibody
    • Para aminohippurate transporter antibody
    • Renal organic anion transporter 1 antibody
    • ROAT1 antibody
    • S22A6_HUMAN antibody
    • SLC22A6 antibody
    • Solute carrier family 22 (organic anion transporter) member 6 antibody
    • Solute carrier family 22 member 6 antibody
    see all

Anti-SLC22A6 antibody 画像

  • Anti-SLC22A6 antibody (ab118346) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 62 kDa
  • ab118346, at 3 µg/ml staining SLC22A6 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • Detection limit for ab118346 is 3 ng/ml as a capture antibody

Anti-SLC22A6 antibody (ab118346) 使用論文

This product has been referenced in:
  • Wu X  et al. Folic acid reverses uric acid crystal-induced surface OAT1 internalization by inhibiting RhoA activity in uric acid nephropathy. Mol Med Rep 13:2385-92 (2016). Read more (PubMed: 26846716) »
  • Topalis D  et al. Resistance to the nucleotide analogue cidofovir in HPV(+) cells: a multifactorial process involving UMP/CMP kinase 1. Oncotarget 7:10386-401 (2016). WB . Read more (PubMed: 26824416) »

See all 3 Publications for this product

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