製品の概要

  • 製品名Anti-SLC22A6 antibody
    SLC22A6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SLC22A6
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 451-550 of Human SLC22A6 with proprietary 26 kDa tag (AAH33682).

  • ポジティブ・コントロール
    • Human kidney tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab118346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23516613
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 62 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
  • 組織特異性Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.
  • 配列類似性Belongs to the major facilitator superfamily. Organic cation transporter family.
  • ドメインMultiple cysteine residues are necessary for proper targeting to the plasma membrane.
  • 翻訳後修飾Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necesssary for proper targeting of the transporter to the plasma membrane.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ55736 antibody
    • hOAT1 antibody
    • hPAHT antibody
    • hROAT1 antibody
    • MGC45260 antibody
    • OAT1 antibody
    • Organic anion transporter 1 antibody
    • OTTHUMP00000236796 antibody
    • OTTHUMP00000236797 antibody
    • OTTHUMP00000236798 antibody
    • OTTHUMP00000236799 antibody
    • PAH transporter antibody
    • PAHT antibody
    • Para aminohippurate transporter antibody
    • Renal organic anion transporter 1 antibody
    • ROAT1 antibody
    • S22A6_HUMAN antibody
    • SLC22A6 antibody
    • Solute carrier family 22 (organic anion transporter) member 6 antibody
    • Solute carrier family 22 member 6 antibody
    see all

Anti-SLC22A6 antibody 画像

  • Anti-SLC22A6 antibody (ab118346) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 62 kDa
  • ab118346, at 3 µg/ml staining SLC22A6 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • Detection limit for ab118346 is 3 ng/ml as a capture antibody

Anti-SLC22A6 antibody (ab118346) 使用論文

This product has been referenced in:
  • DesRochers TM  et al. Bioengineered 3D human kidney tissue, a platform for the determination of nephrotoxicity. PLoS One 8:e59219 (2013). IHC-P, ICC/IF ; Human . Read more (PubMed: 23516613) »

See 1 Publication for this product

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