保存方法 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
精製度 IgG fraction
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 10
6 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use a concentration of 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
機能 Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
組織特異性 Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
配列類似性 Belongs to the major facilitator superfamily. Organic cation transporter family.
Information by UniProt
EMT organic cation transporter 3 antibody
Anti-SLC22A3 antibody [mAbcam75402] 画像
Western blot - SLC22A3 antibody [mAbcam75402] (ab75402)
Anti-SLC22A3 antibody [mAbcam75402] (ab75402) at 5 µg/ml + Human prostate tissue lysate (cirrhosis) - total protein ( ab30307) at 20 µg Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed ( ab97040) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 61 kDa Observed band size : 61 kDa Exposure time : 16 minutes
Flow Cytometry - Anti-SLC22A3 antibody [mAbcam75402] (ab75402)
Overlay histogram showing JEG3 cells stained with ab75402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75402, 1µg/1x10 6 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) ( ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] ( ab91366, 2µg/1x10 6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-SLC22A3 antibody [mAbcam75402] (ab75402) 使用論文
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"