製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC20A2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: WQLIASFLRL PISGTHCIVG STIGFSLVAI GTKGVQWMEL VKIVASWFIS, corresponding to amino acids 101-150 of Human SLC20A2

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能
    Sodium-phosphate symporter which seems to play a fundamental housekeeping role in phosphate transport by absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. In vitro, sodium-dependent phosphate uptake is not siginificantly affected by acidic and alkaline conditions, however sodium-independent phosphate uptake occurs at acidic conditions. May play a role in extracellular matrix, cartilage and vascular calcification. Functions as a retroviral receptor and confers human cells susceptibility to infection to amphotropic murine leukemia virus (A-MuLV), 10A1 murine leukemia virus (10A1 MLV) and some feline leukemia virus subgroup B (FeLV-B) variants.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gibbon ape leukemia virus receptor 2 antibody
    • Gibbon leukemia virus receptor 2 antibody
    • GLVR 2 antibody
    • GLVR-2 antibody
    • GLVR2 antibody
    • hPit2 antibody
    • IBGC3 antibody
    • MLVAR antibody
    • Murine leukemia virus amphotropic receptor antibody
    • murine leukemia virus, amphotropic, receptor for antibody
    • Phosphate transporter 2 antibody
    • PIT 2 antibody
    • PiT-2 antibody
    • Pit2 antibody
    • RAM1 antibody
    • S20A2_HUMAN antibody
    • SLC20A2 antibody
    • Sodium-dependent phosphate transporter 2 antibody
    • Solute carrier family 20 (phosphate transporter) member 2 antibody
    • solute carrier family 20 (phosphate transporter), member 2 antibody
    • Solute carrier family 20 member 2 antibody
    see all

画像

  • Anti-SLC20A2 antibody (ab64412) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab64412 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Ovary)
Permeabilization
No
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 29 2015

Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Cell lysate - whole cell (aortic valve interstitial cells)
Specification
aortic valve interstitial cells
Treatment
Nah2po4 3.5 mM
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jun 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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