製品の概要

  • 製品名Anti-SLC1A5 antibody
    SLC1A5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SLC1A5
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Cow, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human SLC1A5.

    (Peptide available as ab97988.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Jurkat whole cell lysate.
  • 特記事項immunogen sequence is aa244-265 (ALRKLGPEGELLIRFFNSFNEA

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).

ターゲット情報

  • 機能Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. May also be activated by insulin. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904). Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses (PubMed:10051606, PubMed:10196349).
  • 組織特異性Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.
  • 配列類似性Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.
  • 細胞内局在Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ASCT2 antibody
    • AAAT antibody
    • AAAT_HUMAN antibody
    • ATB(0) antibody
    • ATBO antibody
    • Baboon M7 virus receptor antibody
    • FLJ31068 antibody
    • M7V1 antibody
    • M7VS1 antibody
    • Neutral amino acid transporter B(0) antibody
    • R16 antibody
    • RD114/simian type D retrovirus receptor antibody
    • RDR antibody
    • RDRC antibody
    • SLC1A5 antibody
    • Sodium dependent neutral amino acid transporter type 2 antibody
    • Sodium-dependent neutral amino acid transporter type 2 antibody
    • Solute carrier family 1 (neutral amino acid transporter), member 5 antibody
    • Solute carrier family 1 member 5 antibody
    see all

Anti-SLC1A5 antibody 画像

  • Anti-SLC1A5 antibody (ab84903) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,97 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab84903 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84903, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of SLC1A5 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84903, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-SLC1A5 antibody (ab84903) 使用論文

ab84903 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (gel 10%)
Sample Cow Tissue lysate - whole (small intestine)
Specification small intestine
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Miss. xinbei huang

Verified customer

投稿 Jul 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"