製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC17A5
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • Human skeletal muscle tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human SLC17A5 peptide (ab178604). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO(3)(-)/H(+) cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva.
  • 組織特異性
    Found in fetal lung and small intestine, and at lower level in fetal skin and muscle. In the adult, detected in placenta, kidney and pancreas. Abundant in the endothelial cells of tumors from ovary, colon, breast and lung, but is not detected in endothelial cells from the corresponding normal tissues.
  • 関連疾患
    Salla disease
    Infantile sialic acid storage disorder
  • 配列類似性
    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.
  • 細胞内局在
    Cell membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Lysosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AST antibody
    • H(+)/nitrate cotransporter antibody
    • H(+)/sialic acid cotransporter antibody
    • ISSD antibody
    • Membrane glycoprotein HP59 antibody
    • NSD antibody
    • S17A5_HUMAN antibody
    • SD antibody
    • Sialic acid storage disease antibody
    • Sialin antibody
    • SIASD antibody
    • Slc17a5 antibody
    • SLD antibody
    • Solute carrier family 17 (anion/sugar transportermember 5 antibody
    • Solute carrier family 17 member 5 antibody
    • Vesicular H(+)/Aspartate-glutamate cotransporter antibody
    see all

画像

  • Anti-SLC17A5 antibody (ab69819) at 0.5 µg/ml + Transfected 293T cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of paraffin embedded human muscle tissue labelled with ab69819 at 8 µg/ml. Positively labelled skeletal cells are indicated with arrows. Magnification 400X.

参考文献

This product has been referenced in:
  • Richards DS  et al. Motor axon synapses on renshaw cells contain higher levels of aspartate than glutamate. PLoS One 9:e97240 (2014). Read more (PubMed: 24816812) »

See 1 Publication for this product

レビューと Q&A

Thank you for contacting Abcam regarding ab69819.


The immunogen sequence is XXX.

I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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