製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC16A8
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A 14-22 aa region within synthetic peptide: FAVYAVTKFL MALGLFVPAI LLVNYAKDAG VPDTDAAFLL SIVGFVDIVA, corresponding to internal sequence amino acids 253-302 of Human SLC16A8

  • ポジティブ・コントロール
    • Jurkat cell lysate (WB); Human kidney tissue (IHC-P).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性
    SLC16A8 is proton-linked monocarboxylate transporter which catalyzes the rapid transport across the plasma membrane of monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, as well as the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
  • 細胞内局在
    Membrane. Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • MCT 3 antibody
    • MCT3 antibody
    • Monocarboxylate transporter 3 antibody
    • Monocarboxylic acid transporter 3 antibody
    • OTTHUMP00000028510 antibody
    • REMP antibody
    • Solute carrier 16 (monocarboxylic acid transporters) member 8 antibody
    • Solute carrier family 16 member 8 antibody
    • solute carrier family 16, member 8 (monocarboxylic acid transporter 3) antibody
    see all

画像

  • Anti-SLC16A8 antibody (ab60333) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC26A8 with ab60333 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

参考文献

This product has been referenced in:
  • Garnett JP  et al. Hyperglycaemia and Pseudomonas aeruginosa acidify cystic fibrosis airway surface liquid by elevating epithelial monocarboxylate transporter 2 dependent lactate-H(+) secretion. Sci Rep 6:37955 (2016). ICC/IF ; Human . Read more (PubMed: 27897253) »
  • Eilertsen M  et al. Monocarboxylate transporters 1-4 in NSCLC: MCT1 is an independent prognostic marker for survival. PLoS One 9:e105038 (2014). IHC-P ; Human . Read more (PubMed: 25225794) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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