製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SLC16A3
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from internal region of human SLC16A3.

  • ポジティブ・コントロール
    • Extracts from LOVO cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
ELISA 1/40000.

ターゲット情報

  • 機能
    Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
  • 組織特異性
    Highly expressed in skeletal muscle.
  • 配列類似性
    Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains.
  • 別名
    • MCT 4 antibody
    • MCT3 antibody
    • MCT4 antibody
    • Monocarboxylate transporter 3 antibody
    • Monocarboxylate transporter 4 antibody
    • MOT4_HUMAN antibody
    • SLC16A3 antibody
    • Solute carrier family 16 member 3 antibody
    see all

画像

  • All lanes : Anti-SLC16A3 antibody (ab74109) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 49 kDa
    Observed band size : 49 kDa

プロトコール

参考文献

This product has been referenced in:
  • Ghorbel MT  et al. Changes in renal medulla gene expression in a pre-clinical model of post cardiopulmonary bypass acute kidney injury. BMC Genomics 15:916 (2014). Read more (PubMed: 25331815) »

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - whole (Breast)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Breast
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% 37.5:1)
Sample
Pig Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
Thermo Starting Block 37542 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Dec 10 2013

Thank you for providing that information. It allows me to more fully understand the results that you have been observing.

There are a few further things I would like to clarify:

You mentioned that the anti-MCT1 has also been proving...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties withtwo of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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