Anti-SLC12A1/NKCC2 抗体 (ab91098)
Key features and details
- Rabbit polyclonal to SLC12A1/NKCC2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SLC12A1/NKCC2 antibody
SLC12A1/NKCC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLC12A1/NKCC2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Orangutan -
免疫原
Synthetic peptide corresponding to Human SLC12A1/NKCC2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101074) -
ポジティブ・コントロール
- This antibody gave a positive signal in Human kidney tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91098の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa). |
ターゲット情報
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機能
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. -
組織特異性
Kidney specific. -
関連疾患
Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia. -
配列類似性
Belongs to the SLC12A transporter family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6557 Human
- Entrez Gene: 20495 Mouse
- Entrez Gene: 100328575 Rabbit
- Omim: 600839 Human
- SwissProt: Q13621 Human
- SwissProt: P55014 Mouse
- SwissProt: P55015 Rabbit
- Unigene: 123116 Human
see all -
別名
- BSC1 antibody
- Bumetanide sensitive sodium 3 antibody
- Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
see all
画像
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Anti-SLC12A1/NKCC2 antibody (ab91098) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesSLC12A1/NKCC2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab91098 は論文での使用が確認できていません。