Slap overexpression 293T lysate (whole cell) (ab94185)

製品の概要

  • 製品名Slap overexpression 293T lysate (whole cell)
  • 製品の詳細
    Slap overexpression lysate
  • 特記事項ab94185 is a 293T cell transfected lysate in which Human Slap has been transiently over-expressed using a pCMV-Slap plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファーpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度Whole Cell Lysate
  • 研究分野
  • 背景Function: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins. Tissue specificity: Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas. Similarity: Contains 1 SH2 domain. Contains 1 SH3 domain. Domain: The C-terminal domain is essential for the homodimerization and the interaction with CBL. While the interaction with CBL is apparently mediated via the hydrophobic region of this domain, the highly charged region is apparently required for the homodimerization.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab94185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

Slap overexpression 293T lysate (whole cell) 画像

  • ab94185 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Slap antibody (ab58290) at 1/500 dilution

    Lane 1 : Slap overexpression 293T lysate (whole cell) (ab94185)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Slap overexpression 293T lysate (whole cell) (ab94185) 使用論文

ab94185 has not yet been referenced specifically in any publications.

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