製品の概要

  • 製品名
    Anti-SLAM / CD150 antibody [IPO-3]
    SLAM / CD150 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [IPO-3] to SLAM / CD150
  • 特異性
    This antibody is specific for SLAM.
  • アプリケーション
    適用あり: ICC/IF, ELISA, IHC-Fr, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Activated B cell line

  • エピトープ
    The exact epitope recognised by this antibody has not been mapped.
  • 特記事項


    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    PBS pH 7.2, 0.01% sodium azide
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 一次抗体 備考
    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.
  • ポリ/モノ
    モノクローナル
  • クローン名
    IPO-3
  • ミエローマ
    unknown
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21379338
ELISA 1/750.
IHC-Fr 1/100.
Flow Cyt 1/750.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
  • 組織特異性
    Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
  • 配列類似性
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • ドメイン
    The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.
  • 翻訳後修飾
    Phosphorylated by FYN.
  • 細胞内局在
    Cell membrane. Present on the surface of B-cells and T-cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4933415F16 antibody
    • AA177906 antibody
    • CD 150 antibody
    • CD150 antibody
    • CD150 antigen antibody
    • CDw150 antibody
    • Estm51 antibody
    • Ipo 3 antibody
    • IPO-3 antibody
    • Ipo3 antibody
    • MGC151472 antibody
    • MGC151476 antibody
    • OTTHUMP00000025670 antibody
    • OTTHUMP00000060252 antibody
    • RGD1560634 antibody
    • Signaling lymphocytic activation molecule antibody
    • Signaling lymphocytic activation molecule family member 1 antibody
    • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
    • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
    • Signaling lymphocytic activation molecule precursor antibody
    • Slaf1 antibody
    • SLAF1_HUMAN antibody
    • SLAM family, member 1 antibody
    • Slamf 1 antibody
    • SLAMF1 antibody
    • SLAMF1 protein antibody
    see all

画像

  • ab2604, staining SLAM / CD150 in Raji cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100 before being incubated with primary antibody. A fluorescence-conjugated anti-mouse IgG was used as the secondary antibody.

参考文献

This product has been referenced in:
  • Currier MG  et al. EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622 (2016). Read more (PubMed: 27152417) »
  • Avota E  et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . Read more (PubMed: 21379338) »

See all 3 Publications for this product

レビューと Q&A



You are correct the calculated molecular weight of SLAM is ca 37 kDa.

Modification can significantly increase the molecular weight of proteins. SLAM is a sialylated phosphoglycoprotein.

The effect the glycosylation has on ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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