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RabMAb

Anti-SKP2 抗体 [EPR3306(2)] (ab124799)

製品の概要

  • 製品名
    Anti-SKP2 antibody [EPR3306(2)]
    SKP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3306(2)] to SKP2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to residues in Human SKP2.

  • ポジティブ・コントロール
    • 293T, HeLa, SW480 and LnCaP cell lysates; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa).
ICC/IF 1/50 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能
      Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Contains 1 F-box domain.
      Contains 9 LRR (leucine-rich) repeats.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CDK2/Cyclin A associated protein p45 antibody
      • Cyclin A/CDK2 associated protein p45 antibody
      • Cyclin-A/CDK2-associated protein p45 antibody
      • F box protein Skp2 antibody
      • F box/LRR repeat protein 1 antibody
      • F-box protein Skp2 antibody
      • F-box/LRR-repeat protein 1 antibody
      • FBL 1 antibody
      • FBL1 antibody
      • FBXL 1 antibody
      • FBXL1 antibody
      • FLB 1 antibody
      • FLB1 antibody
      • MGC1366 antibody
      • p45 antibody
      • p45skp2 antibody
      • S phase kinase associated protein 2 (p45) antibody
      • S phase kinase associated protein 2 antibody
      • S-phase kinase-associated protein 2 antibody
      • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
      • SKP 2 antibody
      • Skp2 antibody
      • SKP2_HUMAN antibody
      see all

    画像

    • All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : LnCaP cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46, 48 kDa
    • ab124799, at a 1/50 dilution, staining SKP2 in HeLa cells by Immunofluorescence.

    参考文献

    ab124799 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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