製品の概要

  • 製品名Anti-SKP2 antibody
    SKP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SKP2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide raised within aa: 1-50 of human SKP2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP Use at 10-15 µg/mg of lysate.

ターゲット情報

  • 機能Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 F-box domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDK2/Cyclin A associated protein p45 antibody
    • Cyclin A/CDK2 associated protein p45 antibody
    • Cyclin-A/CDK2-associated protein p45 antibody
    • F box protein Skp2 antibody
    • F box/LRR repeat protein 1 antibody
    • F-box protein Skp2 antibody
    • F-box/LRR-repeat protein 1 antibody
    • FBL 1 antibody
    • FBL1 antibody
    • FBXL 1 antibody
    • FBXL1 antibody
    • FLB 1 antibody
    • FLB1 antibody
    • MGC1366 antibody
    • p45 antibody
    • p45skp2 antibody
    • S phase kinase associated protein 2 (p45) antibody
    • S phase kinase associated protein 2 antibody
    • S-phase kinase-associated protein 2 antibody
    • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
    • SKP 2 antibody
    • Skp2 antibody
    • SKP2_HUMAN antibody
    see all

Anti-SKP2 antibody 画像

  • Lane 1: ab87809 (1µg/ml) immunoprecipitating SKP2 in HeLa Whole Cell Lysate (1mg for IP, 20% loaded).
    Lane 2: positive control IP (done using an antibody that recognises downstream epitope).
    Lane 3: Control IgG
  • All lanes : Anti-SKP2 antibody (ab87809) at 0.1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg


    Predicted band size : 48 kDa

Anti-SKP2 antibody (ab87809) 使用論文

ab87809 has not yet been referenced specifically in any publications.

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The anti-SKP2 antibody (ab87809) has been used with HeLa whole cell lysate in order to detect the SKP2 protein. If your customer would like to purchase this as a pre-prepared lysate this can be purchased under the...

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