製品の概要

  • 製品名Anti-SKP2 antibody
    SKP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SKP2
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SKP2.

    (Peptide available as ab26153.)

  • ポジティブ・コントロール
    • Human heart tissue lysate This antibody gave a positive signal in the following formaldehyde fixed cell line: HeLa

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab19877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 F-box domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDK2/Cyclin A associated protein p45 antibody
    • Cyclin A/CDK2 associated protein p45 antibody
    • Cyclin-A/CDK2-associated protein p45 antibody
    • F box protein Skp2 antibody
    • F box/LRR repeat protein 1 antibody
    • F-box protein Skp2 antibody
    • F-box/LRR-repeat protein 1 antibody
    • FBL 1 antibody
    • FBL1 antibody
    • FBXL 1 antibody
    • FBXL1 antibody
    • FLB 1 antibody
    • FLB1 antibody
    • MGC1366 antibody
    • p45 antibody
    • p45skp2 antibody
    • S phase kinase associated protein 2 (p45) antibody
    • S phase kinase associated protein 2 antibody
    • S-phase kinase-associated protein 2 antibody
    • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
    • SKP 2 antibody
    • Skp2 antibody
    • SKP2_HUMAN antibody
    see all

Anti-SKP2 antibody 画像

  • All lanes : Anti-SKP2 antibody (ab19877) at 1 µg/ml

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Human kidney normal tissue lysate
    Lane 4 : Human testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab19877 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab19877 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SKP2 antibody (ab19877) 使用論文

This product has been referenced in:
  • Kelly-Spratt KS  et al. Inhibition of PI-3K restores nuclear p27Kip1 expression in a mouse model of Kras-driven lung cancer. Oncogene 28:3652-62 (2009). WB . Read more (PubMed: 19648963) »

See 1 Publication for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab19877.

To check the status of the order please contact o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U87MG tumour cell line)
Loading amount 50 µg
Specification U87MG tumour cell line
Treatment U87MG cells treated with LY294002 for 6h
Gel Running Conditions Reduced Denaturing (10% tris glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Sep 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela HEK293 MCF7)
Loading amount 20 µg
Specification Hela HEK293 MCF7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Feb 11 2009

Thank you for your reply. I have asked our accounts department to arrange this credit note to pay for the order. I hope this unfortunate matter will not deter you from purchasing at Abcam in the future. Please be assured that I will closely monitor...

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Thank you very much for your reply and detailed protocol which is very similar to the one we recommend. In our experience too much blocking can prevent the antibody from binding adequately to the membrane unfortunately. I would therefore recommend...

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I'm very sorry to hear you are experiencing problems with ab19877. I take your comments very seriously and would appreciate if you could please clarify the conditions you have used the antibody in. It is clear that your samples express high levels o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"