製品の概要

  • 製品名Anti-Skp1 antibody [EPR3304]
    Skp1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3304] to Skp1
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues of human SKP1

  • ポジティブ・コントロール
    • 293T, HeLa, PC-12 and NIH/3T3 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. Use at an assay dependent dilution.
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the SKP1 family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cyclin A/CDK2 associated p19 antibody
      • Cyclin A/CDK2 associated protein p19 antibody
      • Cyclin-A/CDK2-associated protein p19 antibody
      • EMC19 antibody
      • MGC34403 antibody
      • OCP 2 antibody
      • OCP II antibody
      • OCP II protein antibody
      • OCP-2 antibody
      • OCP-II antibody
      • OCP2 antibody
      • OCPII antibody
      • Organ of Corti protein 2 antibody
      • Organ of Corti protein II antibody
      • OTTHUMP00000159379 antibody
      • OTTHUMP00000159380 antibody
      • OTTHUMP00000228275 antibody
      • OTTHUMP00000228276 antibody
      • OTTHUMP00000228278 antibody
      • OTTHUMP00000228279 antibody
      • OTTHUMP00000228281 antibody
      • p19A antibody
      • p19skp1 antibody
      • RNA polymerase II elongation factor like protein antibody
      • RNA polymerase II elongation factor like protein OCP2 antibody
      • RNA polymerase II elongation factor-like protein antibody
      • S phase kinase associated protein 1 antibody
      • S phase kinase associated protein 1A antibody
      • S phase kinase associated protein 1A p19A antibody
      • S-phase kinase-associated protein 1 antibody
      • SIII antibody
      • Skp 1 antibody
      • SKP 1A antibody
      • skp1 antibody
      • SKP1_HUMAN antibody
      • SKP1A antibody
      • TCEB1L antibody
      • Transcription Elongation Factor B 1 like antibody
      • Transcription elongation factor B antibody
      • Transcription elongation factor B SIII polypeptide 1 like antibody
      see all

    Anti-Skp1 antibody [EPR3304] 画像

    • Overlay histogram showing HeLa cells stained with ab76502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labeled IgG at 1/2000 dilution

      Predicted band size : 19 kDa
      Observed band size : 19 kDa
    • ab76502 staining Skp1 in HeLa cells by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed in methanol, blocked with 1% BSA for 30 minutes at 24°C then incubated with ab76502 at a 1/500 dilution for 1 hour at 24°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

      See Abreview

    Anti-Skp1 antibody [EPR3304] (ab76502) 使用論文

    This product has been referenced in:
    • Kainulainen M  et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol 88:3464-73 (2014). WB . Read more (PubMed: 24403578) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"