Synthetic peptide within Human Skp1. The exact sequence is proprietary.
WB: lysates from 293, HeLa, PC12 or 3T3 cells;
IHC-P: Human brain tissue;
FC: permeabilized HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/50.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/1000 - 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Wang D et al. Inhibition of S-phase kinase-associated protein 2 (Skp2) reprograms and converts diabetogenic T cells to Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A109:9493-8 (2012).
Read more (PubMed: 22645357) »