製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Skp1
  • 特異性
    This antibody detects a 19kD band in Hela and HEK293 cells. The band can be blocked with the immunising peptide. The antibody shows extensive staining (eg. of lymphoid and epithelial cells), with staining of nuclei and some staining of the cytoplasm, when tested on human tonsil in IHC.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Guinea pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide derived from residues 50 - 150 of Human Skp1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa nuclear lysate as well as the following whole cell lysates: HeLa; HEK293. IF/ICC: MCF7 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 19 kDa (predicted molecular weight: 18.5 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the SKP1 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cyclin A/CDK2 associated p19 antibody
    • Cyclin A/CDK2 associated protein p19 antibody
    • Cyclin-A/CDK2-associated protein p19 antibody
    • EMC19 antibody
    • MGC34403 antibody
    • OCP 2 antibody
    • OCP II antibody
    • OCP II protein antibody
    • OCP-2 antibody
    • OCP-II antibody
    • OCP2 antibody
    • OCPII antibody
    • Organ of Corti protein 2 antibody
    • Organ of Corti protein II antibody
    • OTTHUMP00000159379 antibody
    • OTTHUMP00000159380 antibody
    • OTTHUMP00000228275 antibody
    • OTTHUMP00000228276 antibody
    • OTTHUMP00000228278 antibody
    • OTTHUMP00000228279 antibody
    • OTTHUMP00000228281 antibody
    • p19A antibody
    • p19skp1 antibody
    • RNA polymerase II elongation factor like protein antibody
    • RNA polymerase II elongation factor like protein OCP2 antibody
    • RNA polymerase II elongation factor-like protein antibody
    • S phase kinase associated protein 1 antibody
    • S phase kinase associated protein 1A antibody
    • S phase kinase associated protein 1A p19A antibody
    • S-phase kinase-associated protein 1 antibody
    • SIII antibody
    • Skp 1 antibody
    • SKP 1A antibody
    • skp1 antibody
    • SKP1_HUMAN antibody
    • SKP1A antibody
    • TCEB1L antibody
    • Transcription Elongation Factor B 1 like antibody
    • Transcription elongation factor B antibody
    • Transcription elongation factor B SIII polypeptide 1 like antibody
    see all

画像

  • ICC/IF image of ab10546 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10546, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18.5 kDa


    Exposure time : 2 minutes

    Western blot using ab10546 at 1/500.

    Lane 1: Hela nuclear
    Lane 2: Hela
    Lane 3: HEK293
    Lane 4: Hela nuclear + ab10546 blocking peptide (1µg/ml)
    Lane 5: Hela + ab10546 blocking peptide (1µg/ml)
    Lane 6: HEK293 + ab10546 blocking peptide (1µg/ml)

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 2 mins.

  • ab10546 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).
    Strong staining of epithelial cell nuclei is seen (and some staining of the cytoplasm) in this image.

     

     


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18.5 kDa


    Exposure time : 20 seconds

参考文献

This product has been referenced in:
  • Bruckert WM  et al. Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila. Infect Immun 82:72-82 (2014). Read more (PubMed: 24126522) »
  • Viñas-Castells R  et al. Nuclear ubiquitination by FBXL5 modulates Snail1 DNA binding and stability. Nucleic Acids Res N/A:N/A (2013). WB . Read more (PubMed: 24157836) »

See all 6 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Dog Tissue sections (MDCK pellets in paraffin)
Specification
MDCK pellets in paraffin
Permeabilization
Yes - Tween-20
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 04 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Treatment
100 ´M FAC (for 6 h) and 10 ´M MG132 (for 4 h)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 08 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Nov 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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