The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500.
Epitope retrieval with Tris-EDTA pH9.0 is recommended for formalin-fixed paraffin-embedded cells.
1/2000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
Is unsuitable for IP.
SKIV2L2, or superkiller viralicidic activity 2 like 2, may be involved in pre-mRNA splicing. SKIV2L2 is an exosome-associated protein and may be involved in the recruitment of the exosome to pre-rRNA, to mediate the 3' end processing of 5.8S rRNA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling SKIV2L2 with ab70551 at 1/200 (1µg/ml). Detection: DAB.
Western blot - SKIV2L2 antibody (ab70551)
All lanes : Anti-SKIV2L2 antibody (ab70551) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 118 kDa Observed band size: 118 kDa
IHC image of ab70551 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.