製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SKA3
  • 特異性
    ab91559 is specific for SKA3 and will not crossreact with SKA1 or SKA2.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 13 amino acid peptide from near the C terminal of Human SKA3 (NP_659498).

  • ポジティブ・コントロール
    • Human testis tissue lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab91559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 46 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
    • 配列類似性
      Belongs to the SKA3 family.
    • 細胞内局在
      Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C13orf3 antibody
      • RAMA1 antibody
      • SKA3 antibody
      • SKA3_HUMAN antibody
      • Spindle and kinetochore associated complex subunit 3 antibody
      • Spindle and kinetochore-associated protein 3 antibody
      see all

    画像

    • Lane 1 : Anti-SKA3 antibody (ab91559) at 0.5 µg/ml
      Lane 2 : Anti-SKA3 antibody (ab91559) at 1 µg/ml

      Lane 1 : Human testis tissue lysate
      Lane 2 : Human testis tissue lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 46 kDa

    プロトコール

    参考文献

    ab91559 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you for keeping me updated and sending the images of the results.

    It does look like the results are a little cleaner, though I'm sorry to see that the results are still not as expected. I believe that the two bands in the testis lysate ...

    Read More

    Thank you so much for your reply and for sending the images.

    The images will be very useful for our investigation into the trouble with these antibodies. Please keep me updated about the results with the testis lysate, and let me know if ther...

    Read More

    Thank you for your call today and for letting us know about the trouble with ab118560. I apologize for the trouble with our SKA3 antibodies.

    As we discussed, I'm sending a free of charge vial of ab30257 human testis lysate to use as a positiv...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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