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Synthetic peptide corresponding to Human Six5 aa 257-286 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
APCKSES DGNPTTEDES SRSPEDLERG AAPV
Our Abpromise guarantee covers the use of ab113064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|ICC/IF||1/10 - 1/50.|
|WB||1/1000. Predicted molecular weight: 75 kDa.|
Immunohistochemistry of formalin fixed and paraffin embedded Human liver tissue labelling Six5 with ab113064.
Immunofluorescence of HeLa cells labelling Six5 with ab113064. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab113064 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Six5 immunoreactivity is localized to the cytoplasm.
ab113064 has not yet been referenced specifically in any publications.
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