Anti-SIV1 Nef 抗体 [17] (ab42361)

製品の概要

  • 製品名Anti-SIV1 Nef antibody [17]
  • 製品の詳細
    Mouse monoclonal [17] to SIV1 Nef
  • 特異性Reacts with SIV of mac251 strain.
  • アプリケーション適用あり: ELISA, WB, Radioimmunoprecipitation, ICC/IFmore details
  • 種交差性
    Simian Immunodeficiency Virus 1.
  • 免疫原

    Recombinant Nef protein of SIV1 mac251 strain.

  • エピトープMapped to amino acids 71-80 (PAEEREKLAY).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名17
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab42361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
WB 1/1000.
Radioimmunoprecipitation 1/5000.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性The Nef proteins of HIV-1 and SIV are 27- to 32 -kDa myristoylated proteins that are found in both the membranes and cytosol of infected host cells. While not strictly required for viral infection and replication, attenuation of Nef expression in either HIV-1 or SIV is associated with markedly delayed progression of disease. Simian immunodeficiency virus (SIV) and HIV-1 Nef proteins are related regulatory proteins that share several functions, including the ability to downregulate class I major histocompatibility complex (MHC) and CD4 expression on the cell surface and to alter T-cell-receptor-initiated signal transduction in T cells. Nef is thus probably involved in viral immune evasion mechanisms.
  • 細胞内局在Cell Membrane, Lipid-anchor.
  • 別名
    • 3'ORF antibody
    • F protein antibody
    • Negative factor antibody

Anti-SIV1 Nef antibody [17] (ab42361) 使用論文

ab42361 has not yet been referenced specifically in any publications.

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For ab42361: DISCOUNT CODE: ***

For ab66643: DISCOUNT CODE: ***

Expiration date: August 5th, 2012

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (PBMC)
Loading amount 25 µg
Specification PBMC
Treatment SIV-infected
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. Karppiah Muthumani

Verified customer

投稿 Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"