製品の概要

  • 製品名Anti-SIT antibody [SIT-01]
    SIT 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SIT-01] to SIT
  • 特異性Human SHP2-interacting transmembrane adapter protein (SIT).
  • アプリケーション適用あり: IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant intracellular fragment (Human).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by protein-A affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名SIT-01
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.
  • 組織特異性Specifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level). Expressed in T- and B-cell lymphoma.
  • 翻訳後修飾Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • gp30/40 antibody
    • SHP intracting transmembrane adapter protein antibody
    • SHP2-interacting transmembrane adapter protein antibody
    • Signaling threshold-regulating transmembrane adapter 1 antibody
    • SIT antibody
    • Sit1 antibody
    • SIT1_HUMAN antibody
    • Suppression inducing transmembrane adapter 1 antibody
    • Suppression-inducing transmembrane adapter 1 antibody
    see all

Anti-SIT antibody [SIT-01] 画像

  • IHC image of ab9528 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9528, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SIT antibody [SIT-01] (ab9528) 使用論文

ab9528 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I regret to inform you that blocking peptides are not available for these 3 antibodies. If you have any additional questions, please don't hesitate to contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"