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RabMAb

Anti-SIRT5 抗体 [EPR5411] (ab108968)

製品の概要

  • 製品名
    Anti-SIRT5 antibody [EPR5411]
    SIRT5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5411] to SIRT5
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminus of Human SIRT5 (UniProt Q9NXA8).

  • ポジティブ・コントロール
    • Fetal liver, HeLa and A431 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/10000.

  • 追加情報
    Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    • 機能
      NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
    • 組織特異性
      Widely expressed.
    • 配列類似性
      Belongs to the sirtuin family. Class III subfamily.
      Contains 1 deacetylase sirtuin-type domain.
    • ドメイン
      In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
    • 細胞内局在
      Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NAD dependent deacetylase sirtuin 5 antibody
      • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
      • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
      • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
      • Regulatory protein SIR2 homolog 5 antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
      • Sir2 like 5 antibody
      • SIR2-like protein 5 antibody
      • SIR2L5 antibody
      • SIR5_HUMAN antibody
      • Sirt5 antibody
      • Sirtuin 5 antibody
      • Sirtuin type 5 antibody
      see all

    画像

    • Anti-SIRT5 antibody [EPR5411] (ab108968) at 1/5000 dilution + Human Fetal liver lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

      Predicted band size : 34 kDa
    • All lanes : Anti-SIRT5 antibody [EPR5411] (ab108968) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal liver cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit. at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    プロトコール

    参考文献

    This product has been referenced in:
    • Buler M  et al. SIRT5 is under the control of PGC-1a and AMPK and is involved in regulation of mitochondrial energy metabolism. FASEB J 28:3225-37 (2014). Read more (PubMed: 24687991) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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