Anti-SIRT5 抗体 (ab13697)


  • 製品名Anti-SIRT5 antibody
    SIRT5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SIRT5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 30-46 of Human SIRT5.

  • ポジティブ・コントロール
    • Human rat or mouse intestine tissue lysate.



Our Abpromise guarantee covers the use of ab13697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 36 kDa.


  • 機能NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the sirtuin family. Class III subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • ドメインIn contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).
  • 細胞内局在Mitochondrion; Cytoplasm. Mitochondrion and Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NAD dependent deacetylase sirtuin 5 antibody
    • NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody
    • NAD dependent protein deacylase sirtuin 5 mitochondrial antibody
    • NAD-dependent protein deacylase sirtuin-5, mitochondrial antibody
    • Regulatory protein SIR2 homolog 5 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody
    • Sir2 like 5 antibody
    • SIR2-like protein 5 antibody
    • SIR2L5 antibody
    • SIR5_HUMAN antibody
    • Sirt5 antibody
    • Sirtuin 5 antibody
    • Sirtuin type 5 antibody
    see all

Anti-SIRT5 antibody 画像

  • Anti-SIRT5 antibody (ab13697) at 1/1000 dilution + Human intestine lysate
  • All lanes : Anti-SIRT5 antibody (ab13697) at 2 µg/ml

    Lane 1 : Human intestine cell lysate
    Lane 2 : Mouse intestine cell lysate
    Lane 3 : Rat intestine cell lysate

    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

Anti-SIRT5 antibody (ab13697) 使用論文

ab13697 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab108968 with the order number XXX.


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According to our records, ab13697 was proving difficult to use in WB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand ...

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Thank you for your honest feedback. I agree that the vial of ab13697 you received is not specific for SIRT5. As per our Abpromise guarantee, if you have received this product within the last 6 months, I am happy to offer a free replacement of any prima...

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Application Western blot
Sample Mouse Tissue lysate - other (Liver mitochondrial extract)
Loading amount 20 µg
Specification Liver mitochondrial extract
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step LiCOR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Michelle Green

Verified customer

投稿 Aug 08 2012

Thank you for contacting Abcam regarding ab13697.

I am sorry that this antibody is not working as specified on the datasheet. I am happy to offer a replacement or credit for this product.

Unfortunately, I am not able to send samples o...

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Thank you for contacting us.

Your credit note ID is xxx.

As per our order system, both ab13697 and ab90845 have already been refunded in xxx with credit note ID xxx.

The current credit note is for ab125881. Read More

Thank you for your call today and for letting us know about the trouble with these antibodies.

Your credit note ID is ***.

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made...

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Thank you for providing more details of your customer's protocol, below are my recommendations to increase the signal (those are very similar to my recommendations for the other antibodies the customer is having problems with): -as discussed before...

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Thank you for contacting us for technical support. The fact the customer is having the correct results with over-expressed protein reassures us that the antibody works and that in Hek293T cell lysate the protein levels are much lower. We have not h...

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