製品の概要

  • 製品名Anti-SIRT4 antibody
    SIRT4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to SIRT4
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    SRCGELLPLIDPC

    , corresponding to C terminal amino acids 302-314 of Human SIRT4.

  • ポジティブ・コントロール
    • Human kidney and U937 lysate. Humsn skeletal muscle tissue.
  • 特記事項


    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab10140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human SIRT4 peptide (ab23185).
IHC-P Use a concentration of 2 - 3 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended.

ターゲット情報

  • 機能Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion.
  • 組織特異性Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus.
  • 配列類似性Belongs to the sirtuin family. Class II subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC130046 antibody
    • MGC130047 antibody
    • MGC57437 antibody
    • NAD dependent deacetylase sirtuin 4 antibody
    • NAD dependent protein deacetylase sirtuin 4 antibody
    • NAD-dependent ADP-ribosyltransferase sirtuin-4 antibody
    • NAD-dependent protein deacetylase sirtuin-4 antibody
    • NAD-dependent protein lipoamidase sirtuin-4, mitochondrial antibody
    • Regulatory protein SIR2 homolog 4 antibody
    • Silent mating type information regulation 2 homolog antibody
    • Sir 2 like 4 antibody
    • SIR 2 like protein 4 antibody
    • Sir2 like 4 antibody
    • SIR2 like protein 4 antibody
    • SIR2-like protein 4 antibody
    • SIR2L 4 antibody
    • SIR2L4 antibody
    • SIR4_HUMAN antibody
    • SIRT 4 antibody
    • Sirt4 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 4 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody
    • Sirtuin 4 antibody
    • Sirtuin type 4 antibody
    • Sirtuin-4 antibody
    • Sirtuin4 antibody
    see all

Anti-SIRT4 antibody 画像

  • ab10140 at 2.5µg/ml staining SIRT4 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in steam in citrate buffer pH 6.0. AP staining procedure was used for detection.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, staining SIRT4 with ab10140 at 2 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).
  • Anti-SIRT4 antibody (ab10140) at 0.05 µg/ml + Human kidney lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 36 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SIRT4 antibody (ab10140) at 3 µg/ml

    Lane 1 : MOCK-transfected HEK293 cell lysate
    Lane 2 : SIRT4-transfected HEK293 cell lysate


    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-SIRT4 antibody (ab10140) 使用論文

This product has been referenced in:
  • McGlynn LM  et al. SIRT3 & SIRT7: Potential Novel Biomarkers for Determining Outcome in Pancreatic Cancer Patients. PLoS One 10:e0131344 (2015). IHC ; Human . Read more (PubMed: 26121130) »
  • Shih J  et al. Loss of SIRT4 decreases GLT-1-dependent glutamate uptake and increases sensitivity to kainic acid. J Neurochem N/A:N/A (2014). Read more (PubMed: 25196144) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain (coronal sections))
Specification Brain (coronal sections)
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 15 2013

That is correct. The antibody is goat immunoglobulin, purified from goat serum. The immunogen is derived from human Sirt4, but the antibody itself is only goat IgG. If you or your customer still have any questions, please contact us.

Thanks again for contacting us with your question, and for your patience. The peptide for ab49173 is now available on our website as ab123888- http://www.abcam.com/SIRT5-peptide-ab123888.html Unfortunately the peptide for ab78977 is not available for s...

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Thank you for contacting us with your question. We do have the blocking peptide for the SIRT5 antibody ab49173, but it isn't on our website at this point so I'm working to have this added. I'll have to check with the lab about the peptide for the SIRT7...

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Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s Team who would b...

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Thank you very much for confirming these details. I would be pleased to replace the antibody with an Sirt4 antibody. If the customer is interested in an antibody for IHC-P, I agree that ab10140, ab105039 and ab115517 are suitable. As mentioned on t...

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Thank you for your customer's protocol details, I enclose below my recommendations to increase the signal, these are very similar to those given for the other antibodies the customer is having problems with: -as discussed before the fact the custom...

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Thank you for contacting us for technical support. The fact the customer is having the correct results with over-expressed protein reassures us that the antibody works and that in Hek293T cell lysate the protein levels are much lower. We have not h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"