製品の概要

  • 製品名Anti-SIRT3 antibody
    SIRT3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SIRT3
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SIRT3.

    (Peptide available as ab45066.)

  • ポジティブ・コントロール
    • ab45067 gave a positive signal in the following whole cell lysates: HeLa; HepG2; Hek293.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 翻訳後修飾Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hSIRT 3 antibody
    • hSIRT3 antibody
    • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
    • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
    • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
    • Regulatory protein SIR2 homolog 3 antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
    • Sir 2 like 3 antibody
    • SIR 2 like protein 3 antibody
    • SIR 3 antibody
    • SIR2 L3 antibody
    • Sir2 like 3 antibody
    • SIR2 like protein 3 antibody
    • SIR2-like protein 3 antibody
    • SIR2L3 antibody
    • SIR3_HUMAN antibody
    • SIRT 3 antibody
    • SIRT3 antibody
    • Sirtuin 3 antibody
    • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
    • Sirtuin type 3 antibody
    • Sirtuin3 antibody
    see all

Anti-SIRT3 antibody 画像

  • All lanes : Anti-SIRT3 antibody (ab45067) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • ICC/IF image of ab45067 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab45067, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-SIRT3 antibody (ab45067) 使用論文

This product has been referenced in:
  • Sarin M  et al. Alterations in c-Myc phenotypes resulting from dynamin-related protein 1 (Drp1)-mediated mitochondrial fission. Cell Death Dis 4:e670 (2013). Read more (PubMed: 23764851) »

See 1 Publication for this product

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Thank you for contacting Abcam Scientific Support.

Would you be able to provide me with specifics about the problem which you have been experiencing with ab15734? In particular it would be great to know the application in which the product w...

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Nous avons 2 références correspondant à la SIRT3 :

ab125810, SIRT3 protein (Active + tagged), aa 47-399 + tag, http://www.abcam.com/ab125810
ab125586, SIRT3 protein (Tagged), aa 1-399 + tag (N-term), http:/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"