The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Is unsuitable for IHC-P.
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
Phosphorylated at the G2/M transition of the cell cycle.
Cytoplasm > cytoskeleton. Colocalizes with microtubules.
Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).
This product has been referenced in:
Du Y et al. Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression. Mol Med Rep15:1638-1646 (2017).
Read more (PubMed: 28259910) »
van Leeuwen IM et al. Modulation of p53 C-terminal acetylation by mdm2, p14ARF, and cytoplasmic SirT2. Mol Cancer Ther12:471-80 (2013).
Read more (PubMed: 23416275) »