製品の概要

  • 製品名Anti-SIRT2 antibody
    SIRT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SIRT2
  • 特異性SIRT2 antibody (ab19388) detects SIRT2 and shows no cross-reactivity to other SIRT isoforms.
  • アプリケーション適用あり: WB, ICCmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human SIRT2 aa 377-389.
    Sequence:

    DEARTTEREKPQ

  • ポジティブ・コントロール
    • SAOS2 transfected cell lysates, human chondrocyte lysates.
  • 特記事項


    The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
  • 精製度Whole antiserum
  • 一次抗体 備考The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
ICC 1/400.

ターゲット情報

  • 機能NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • 組織特異性Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • 配列類似性Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • 発生段階Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • 翻訳後修飾Phosphorylated at the G2/M transition of the cell cycle.
  • 細胞内局在Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Anti-SIRT2 antibody 画像

  • Anti-SIRT2 antibody (ab19388) at 1/1000 dilution

    Predicted band size : 43 kDa
  • Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using ab19388.

Anti-SIRT2 antibody (ab19388) 使用論文

This product has been referenced in:
  • Lara E  et al. Salermide, a Sirtuin inhibitor with a strong cancer-specific proapoptotic effect. Oncogene 28:781-91 (2009). WB ; Human . Read more (PubMed: 19060927) »
  • Lovato L  et al. Transketolase and CNPase I are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol Cell Proteomics : (2008). WB ; Human . Read more (PubMed: 18676363) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

Thank you for your reply, the refund will be arranged soon by our accounts department. I'm afraid we do not have the immunogen peptide in our catalogue nor can we source it for you as ab19388 is not made in house, I'm very sorry I cannot help you more,

Read More

I'm sorry to hear you are experiencing problems with ab19388. Many thanks for providing the image of your blot and your detailed protocol, it is very useful for me to understand your problem. I would like to recommend some changes to your protocol ...

Read More

Thank you for contacting us for technical support. The fact the customer is having the correct results with over-expressed protein reassures us that the antibody works and that in Hek293T cell lysate the protein levels are much lower. We have not h...

Read More

Thank you for your email, and I'm replying on behalf of Sarah who is currently away from the office. I'm sorry to hear that ab19388 is still giving you difficulty and I can certainly arrange for a free of charge replacement vial to be sent to you. ...

Read More

Thank you for contacting us for technical support and for taking the time to provide all your protocol details, this was really useful to understand your problem. I suspect the problem is due to the blocking agent present in your antibody dilution b...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"