Anti-SIRT2 抗体 (ab115681)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SIRT2
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide containing 19 amino acids from the C terminal region of Human SIRT2

  • ポジティブ・コントロール
    • Human brain (cerebellum) tissue; Human heart tissue; Human placenta tissue; Mouse brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • 組織特異性
    Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • 配列類似性
    Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • 発生段階
    Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • 翻訳後修飾
    Phosphorylated at the G2/M transition of the cell cycle.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

画像

  • ab115681 at 5ug/ml staining SIRT2 in Human brain (cerebellum) tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115681 at 5ug/ml staining SIRT2 in Human heart tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115681 at 5ug/ml staining SIRT2 in Human placenta tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-SIRT2 antibody (ab115681) at 1 µg/ml
    Lane 2 : Anti-SIRT2 antibody (ab115681) at 2 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse brain lysate


    Predicted band size : 43 kDa

参考文献

ab115681 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us. SIRT6 The human, mouse and rat proteins are homologous in N terminal and central region of protein. So the antibodies available to these regions will most likely cross-react. ab62739, cross reacts with human, mouse an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse fibroblast cell)
Loading amount
50 µg
Specification
mouse fibroblast cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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