Anti-SIRT2 抗体 (ab115681)

製品の概要

  • 製品名Anti-SIRT2 antibody
    SIRT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SIRT2
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide containing 19 amino acids from the C terminal region of Human SIRT2

  • ポジティブ・コントロール
    • Human brain (cerebellum) tissue; Human heart tissue; Human placenta tissue; Mouse brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • 組織特異性Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • 配列類似性Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • 発生段階Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • 翻訳後修飾Phosphorylated at the G2/M transition of the cell cycle.
  • 細胞内局在Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ35621 antibody
    • FLJ37491 antibody
    • NAD dependent deacetylase sirtuin 2 antibody
    • NAD-dependent deacetylase sirtuin-2 antibody
    • NAD-dependent protein deacetylase sirtuin-2 antibody
    • Regulatory protein SIR2 homolog 2 antibody
    • Silencing information regulator 2 like antibody
    • Silent information regulator 2 antibody
    • SIR2 antibody
    • SIR2 like protein 2 antibody
    • Sir2 related protein type 2 antibody
    • SIR2, S. cerevisiae, homolog-loke 2 antibody
    • SIR2-like protein 2 antibody
    • SIR2L antibody
    • SIR2L2 antibody
    • SIRT2 antibody
    • SIRT2_HUMAN antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
    • Sirtuin 2 antibody
    • Sirtuin type 2 antibody
    see all

Anti-SIRT2 antibody 画像

  • ab115681 at 5ug/ml staining SIRT2 in Human brain (cerebellum) tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115681 at 5ug/ml staining SIRT2 in Human heart tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115681 at 5ug/ml staining SIRT2 in Human placenta tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-SIRT2 antibody (ab115681) at 1 µg/ml
    Lane 2 : Anti-SIRT2 antibody (ab115681) at 2 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse brain lysate


    Predicted band size : 43 kDa

Anti-SIRT2 antibody (ab115681) 使用論文

ab115681 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. SIRT6 The human, mouse and rat proteins are homologous in N terminal and central region of protein. So the antibodies available to these regions will most likely cross-react. ab62739, cross reacts with human, mouse an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse fibroblast cell)
Loading amount 50 µg
Specification mouse fibroblast cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"