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Our Abpromise guarantee covers the use of ab12193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50. (determined by indirect immunofluorescent staining of methanol fixed cultured mouse 3T3-NIH cells).|
|WB||1/2000. Predicted molecular weight: 80 kDa. (nuclear extract of mouse 3T3-NIH cells). Observed molecular weight is 110 kDa. In some preparations additional lower bands may be detected.|
Immunocytochemical Immunofluorescence analysis of fixed NIH3T3 cells labelling SIRT1 with ab12193 at a concentration of 20 μg/mL. The secondary used was a Goat Anti-Rabbit IgG, Atto® 488 conjugate. Nuclear staining with DAPI.
Immunocytochemical immunofluorescence analysis of fixed HeLa cells labelling SIRT1 using ab12193 at a concentration of 20 μg/mL. The secondary used was a Goat Anti-Rabbit IgG, Atto® 488 conjugate. Counterstaining was with DAPI against Nuclear DNA.
ab12193 staining SIRT1 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for 60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation of rSCs with RSV led to an increase of SIRT1 expression in densitometry analysis.