Anti-SIRP alpha 抗体 [15-414] (Allophycocyanin) (ab95627)

製品の概要

  • 製品名Anti-SIRP alpha antibody [15-414] (Allophycocyanin)
    SIRP alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [15-414] to SIRP alpha (Allophycocyanin)
  • 標識Allophycocyanin. Ex: 645nm, Em: 660nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human, Monkey
  • 免疫原

    Tissue, cells or virus corresponding to Human SIRP alpha.

  • ポジティブ・コントロール
    • Human peripheral blood cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 5µl for 106 cells. ab91364-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • 組織特異性Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
  • 配列類似性Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾N-glycosylated.
    Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Signal regulatory protein alpha type 1 antibody
    • Bit antibody
    • Brain Ig like molecule with tyrosine based activation motifs antibody
    • Brain Ig-like molecule with tyrosine-based activation motifs antibody
    • Brain immunoglobulin like molecule with tyrosine based activation motifs antibody
    • CD172 antigen like family member A antibody
    • CD172 antigen-like family member A antibody
    • CD172a antibody
    • CD172a antigen antibody
    • Inhibitory receptor SHPS-1 antibody
    • Macrophage fusion receptor antibody
    • MFR antibody
    • MYD 1 antibody
    • Myd 1 antigen antibody
    • MyD-1 antigen antibody
    • p84 antibody
    • Protein tyrosine phosphatase non receptor type substrate 1 antibody
    • PTPNS1 antibody
    • SHP substrate 1 antibody
    • SHPS-1 antibody
    • SHPS1 antibody
    • SHPS1_HUMAN antibody
    • Signal regulatory protein alpha 2 antibody
    • Signal regulatory protein alpha 3 antibody
    • Signal regulatory protein alpha antibody
    • Signal regulatory protein alpha type 2 antibody
    • Signal-regulatory protein alpha-1 antibody
    • Signal-regulatory protein alpha-2 antibody
    • Signal-regulatory protein alpha-3 antibody
    • SIRP antibody
    • Sirp-alpha-1 antibody
    • Sirp-alpha-2 antibody
    • Sirp-alpha-3 antibody
    • SIRPA antibody
    • SIRPalpha antibody
    • SIRPalpha1 antibody
    • SIRPalpha2 antibody
    • SIRPalpha3 antibody
    • Tyrosine phosphatase SHP substrate 1 antibody
    • Tyrosine protein phosphatase non receptor type substrate 1 antibody
    • Tyrosine-protein phosphatase non-receptor type substrate 1 antibody
    see all

Anti-SIRP alpha antibody [15-414] (Allophycocyanin) 画像

  • Staining of normal Human peripheral blood cells with Mouse IgG2a k Isotype Control APC (left) or ab95627 (right). Cells in the lymphocyte (green histogram) or granulocyte (blue histogram) gate were used for analysis

Anti-SIRP alpha antibody [15-414] (Allophycocyanin) (ab95627) 使用論文

ab95627 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (from buffy coat)
Specification from buffy coat
Preparation Cell harvesting/tissue preparation method: after ficoll purification
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 15 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 08 2013

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 08 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Jan 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"