製品の概要

  • 製品名Anti-SIN1 antibody
    SIN1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SIN1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Dog, Human
  • 免疫原

    Synthetic peptide: QIKCKNIQWK ERNSKQSAQE LKSLFEKKSL KEKPPISGKQ SILSVRLEQC P , corresponding to amino acids 100-150 of Human SIN1

  • ポジティブ・コントロール
    • Human, mouse and rat skeletal muscle lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: 0.2% Gelatin, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 59 kDa).

ターゲット情報

  • 機能Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
  • 組織特異性Ubiquitously expressed, with highest levels in heart and skeletal muscle.
  • 配列類似性Belongs to the SIN1 family.
  • 細胞内局在Cell membrane. Cytoplasmic vesicle. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MAPKAP 1 antibody
    • MAPKAP1 antibody
    • MEKK2 interacting protein 1 antibody
    • MGC2745 antibody
    • MIP 1 antibody
    • MIP1 antibody
    • Mitogen activated protein kinase associated protein 1 antibody
    • Mitogen-activated protein kinase 2-associated protein 1 antibody
    • mSIN1 antibody
    • OTTHUMP00000064207 antibody
    • Ras inhibitor MGC2745 antibody
    • SAPK interacting protein 1 antibody
    • SAPK-interacting protein 1 antibody
    • SIN 1 antibody
    • SIN1_HUMAN antibody
    • SIN1b antibody
    • SIN1g antibody
    • Stress activated map kinase interacting protein 1 antibody
    • Stress activated protein kinase interacting 1 antibody
    • Stress-activated map kinase-interacting protein 1 antibody
    • Target of rapamycin complex 2 subunit MAPKAP1 antibody
    • TORC2 subunit MAPKAP1 antibody
    see all

Anti-SIN1 antibody 画像

  • All lanes : Anti-SIN1 antibody (ab72161) at 2 µg/ml

    Lane 1 : human skeletal muscle lysate
    Lane 2 : human skeletal muscle lysate with immunizing peptide
    Lane 3 : mouse skeletal muscle lysate
    Lane 4 : rat skeletal muscle lysate


    Predicted band size : 59 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-SIN1 antibody (ab72161) 使用論文

ab72161 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab72161.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"