製品の概要

  • 製品名Anti-SIN1 antibody
    SIN1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SIN1
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    A synthetic peptide corresponding to a region between residue 470 and the C-terminus (residue 522) of human SIN1 (NP_001006618.1).

  • ポジティブ・コントロール
    • Whole cell lysate from Hela cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 30, 50, 65 kDa (predicted molecular weight: 59 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
  • 組織特異性Ubiquitously expressed, with highest levels in heart and skeletal muscle.
  • 配列類似性Belongs to the SIN1 family.
  • 細胞内局在Cell membrane. Cytoplasmic vesicle. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MAPKAP 1 antibody
    • MAPKAP1 antibody
    • MEKK2 interacting protein 1 antibody
    • MGC2745 antibody
    • MIP 1 antibody
    • MIP1 antibody
    • Mitogen activated protein kinase associated protein 1 antibody
    • Mitogen-activated protein kinase 2-associated protein 1 antibody
    • mSIN1 antibody
    • OTTHUMP00000064207 antibody
    • Ras inhibitor MGC2745 antibody
    • SAPK interacting protein 1 antibody
    • SAPK-interacting protein 1 antibody
    • SIN 1 antibody
    • SIN1_HUMAN antibody
    • SIN1b antibody
    • SIN1g antibody
    • Stress activated map kinase interacting protein 1 antibody
    • Stress activated protein kinase interacting 1 antibody
    • Stress-activated map kinase-interacting protein 1 antibody
    • Target of rapamycin complex 2 subunit MAPKAP1 antibody
    • TORC2 subunit MAPKAP1 antibody
    see all

Anti-SIN1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SIN1 with ab71152 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SIN1 antibody (ab71152) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg


    Predicted band size : 59 kDa
    Observed band size : 60,65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of SIN1
    Lane 1: ab71152 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG IP.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed with 1 µg/ml ab71152.
    Chemiluminescence with an exposure time of 30 seconds.

Anti-SIN1 antibody (ab71152) 使用論文

This product has been referenced in:
  • González-Barriga A  et al. Design and analysis of effects of triplet repeat oligonucleotides in cell models for myotonic dystrophy. Mol Ther Nucleic Acids 2:e81 (2013). WB ; Mouse, Human . Read more (PubMed: 23511335) »
  • Joha S  et al. GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells. Oncogene : (2011). Read more (PubMed: 21804606) »

See all 2 Publications for this product

Product Wall

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