製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SIKE1
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 27-103 (ESLVDQSAAL HRRVAAMREA GTALPDQYQE DASDMKDMSK YKPHILLSQE NTQIRDLQQE NRELWISLEE HQDALEL) of Human SIKE1.

  • ポジティブ・コントロール
    • Human small intestine tissue; Transfected HEK293T cell lysate; U-251 MG cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
  • 組織特異性
    Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).
  • 配列類似性
    Belongs to the SIKE family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ21168 antibody
    • RGD1311316 antibody
    • RP5-1000E10.4 antibody
    • SIKE antibody
    • sike1 antibody
    • SIKE1_HUMAN antibody
    • Suppressor of IKBKE 1 antibody
    • Suppressor of IKK-epsilon antibody
    see all

画像

  • ab121795, at 1/325 dilution, staining SIKE1 in paraffin-embedded Human small intestine tissue, by Immunohistochemistry.
  • All lanes : Anti-SIKE1 antibody (ab121795) at 1/250 dilution

    Lane 1 : Negative control (Vector-only transfected HEK293T lysate).
    Lane 2 : SIKE1-transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique
  • ab121795, at 4 µg/ml, staining SIKE1 (green) in Human U-251 MG cells, by Immunofluorescence.

参考文献

ab121795 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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