The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Use at an assay dependent concentration. PubMed: 21573736
Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration.
Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells.
Amyotrophic lateral sclerosis 16, juvenile
Belongs to the ERG2 family.
Nucleus inner membrane. Nucleus outer membrane. Endoplasmic reticulum membrane. Lipid droplet. Cell junction. Cell membrane. Cell projection > growth cone. Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum. Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding.
Western blot - Anti-Sigma1-receptor antibody (ab53852)
All lanes : Anti-Sigma1-receptor antibody - C-terminal (ab53852) at 2 µg/ml
Lane 1 : Mouse brain homogenate Lane 2 : Rat liver homogenate Lane 3 : Rat brain homogenate
Predicted band size : 25 kDa Observed band size : 25 kDa Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry - Free Floating - Anti-Sigma1-receptor antibody (ab53852)This image is courtesy of an Abreview submitted by Marco Peviani.
ab53852 staining Sigma1-receptor in mouse lumbar spinal cord tissue (30 micron sections) by Immunohistochemistry (Free-floating sections). Blocking was done by incubating the sections for 1 hour at room temperature in NGS 10% + Triton 0.1% in PBS. The primary antibody was diluted 1/250 in 3% goat serum in PBS and incubated with the sample for 16 hours at 4°C. An Alexa fluor® 647-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500. Sections were also immunostained with Hoechst dye (blue) to highlight nuclei. The green signal is GFP expressed in motor neuronal cells.
Shinjo S et al. Disruption of the mitochondria-associated ER membrane (MAM) plays a central role in palmitic acid-induced insulin resistance. Exp Cell Res359:86-93 (2017).
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