製品の概要

  • 製品名Anti-Sigma1-receptor antibody
    Sigma1-receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sigma1-receptor
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Sigma1-receptor (internal sequence).
    Database link: Q99720

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21573736
WB 1/200 - 1/2000. Predicted molecular weight: 25 kDa.
IHC-P 1/50 - 1/500.

ターゲット情報

  • 機能Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration.
  • 組織特異性Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells.
  • 関連疾患Amyotrophic lateral sclerosis 16, juvenile
  • 配列類似性Belongs to the ERG2 family.
  • 細胞内局在Nucleus inner membrane. Nucleus outer membrane. Endoplasmic reticulum membrane. Lipid droplet. Cell junction. Cell membrane. Cell projection > growth cone. Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum. Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding.
  • Information by UniProt
  • 参照データベース
  • 別名
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Anti-Sigma1-receptor antibody (ab89655) 使用論文

This product has been referenced in:
  • Hayashi T  et al. Antigen retrieval to improve the immunocytochemistry detection of sigma-1 receptors and ER chaperones. Histochem Cell Biol 135:627-37 (2011). WB, ICC/IF ; Chinese Hamster . Read more (PubMed: 21573736) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293 cells)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 200 µg
Specification 293 cells
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Marina Shenkman

Verified customer

投稿 Jun 02 2015

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Mouse Tissue lysate - whole (mECK36)
Specification mECK36
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Dec 04 2013

I regret to inform you that I am unable to release any information regarding the immunogen used.

Apologies!

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of alternative ab89655 has been...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ...

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Thank you for your reply and for kindly providing the images.

Before proceeding, I would like to ensure I am understanding the results correctly from the images provided. I would appreciate if you could confirm the following:

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