製品の概要

  • 製品名
    Anti-SIGLEC5 antibody [1A5]
    SIGLEC5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1A5] to SIGLEC5
  • 特異性
    This antibody cross reacts with SEGLEC14.
  • アプリケーション
    適用あり: IHC-Fr, ELISA, Flow Cyt, ICC/IF, IP, Neutralising, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Siglec-5-Fc protein, containing entire extracellular region of siglec-5 fused with the Fc region of human IgG1.

  • ポジティブ・コントロール
    • Siglec 5 transfected CHO cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    1A5
  • ミエローマ
    Sp2
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.

ターゲット情報

  • 機能
    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
  • 組織特異性
    Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • ドメイン
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD170 antibody
    • CD170 antigen antibody
    • CD33 antigen-like 2 antibody
    • CD33L2 antibody
    • mSiglec-F antibody
    • OB-binding protein 2 antibody
    • OB-BP2 antibody
    • OBBP2 antibody
    • Obesity-binding protein 2 antibody
    • Sialic acid binding Ig-like lectin 5 antibody
    • Sialic acid binding immunoglobulin-like lectin 5 antibody
    • Sialic acid-binding Ig-like lectin 5 antibody
    • Sialic acid-binding Ig-like lectin F antibody
    • SIGL5_HUMAN antibody
    • Siglec-5 antibody
    • Siglec-F variant antibody
    • Siglec5 antibody
    • Siglecf antibody
    see all

画像

  • Overlay histogram showing THP1 cells stained with ab97426 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97426, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

参考文献

This product has been referenced in:
  • Cornish AL  et al. Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33. Blood 92:2123-32 (1998). Read more (PubMed: 9731071) »

See 1 Publication for this product

レビューと Q&A

Thank you for your inquiry.
Unfortunately, the immunogen of ab97426 only shows 50% homology with mouse and we therefore would not expect a cross reaction.
ab38582 is tested and guaranteed for mouse samples. Maybe this one is suitable for your...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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