製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SIDT1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    RFQRKSIDGSFGSN

    by a Cysteine residue linker, corresponding to internal sequence amino acids 334-347 of Human SIDT1 (NP_060169.2).

  • ポジティブ・コントロール
    • Human cerebellum lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

ターゲット情報

画像

  • All lanes : Anti-SIDT1 antibody (ab123808) at 0.300000011921 µg/ml

    Lane 1 : Human spleen lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Mouse thymus lysate
    Lane 5 : Rat thymus lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 94 kDa
  • Anti-SIDT1 antibody (ab123808) at 0.3 µg/ml (incubation time: 1 hour) + Human cerebellum lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 94 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab123808 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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