Anti-Sialyl Tn 抗体 [STn 219] (FITC) (ab76756)

製品の概要

  • 製品名Anti-Sialyl Tn antibody [STn 219] (FITC)
    Sialyl Tn 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [STn 219] to Sialyl Tn (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性ab76756 stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
  • アプリケーション適用あり: Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Sheep, Human
  • 免疫原

    Ovine submaxillary mucin (OSM)

  • エピトープNeuAc alpha GalNacOSer/Thr.
  • ポジティブ・コントロール
    • Human gastrointestinal tumor, prostate and ovary carcinomas.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2.5µl for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 - 20 µg/ml.

ターゲット情報

  • 関連性Sialyl-Tn is a carbohydrate antigen overexpressed in several epithelial cancers including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase: ST6GalNAc I, which catalyzes the transfer of a sialic acid residue in alpha2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure. The resulting disaccharide (Neu5Acalpha2-6GalNAcalpha1-O-Ser/Thr) cannot be further elongated and sialyl-Tn expression results therefore in a shortening of the O-glycan chains
  • 別名
    • CD175s antibody
    • Sialosyl Tn antibody
    • sTn antibody

Anti-Sialyl Tn antibody [STn 219] (FITC) 画像

  • Overlay histogram showing DU145 cells stained with ab76756 (red line). The cells were fixed with 80%methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76756, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

Anti-Sialyl Tn antibody [STn 219] (FITC) (ab76756) 使用論文

ab76756 has not yet been referenced specifically in any publications.

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