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Our Abpromise guarantee covers the use of ab18619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20696778|
|WB||1/250. 1/250 (see Abreview).|
|RIA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. Fix with acetone.|
ab18619 staining human peripheral blood mononuclear cells by flow cytometry. The cells were prepared in FACS buffer (PBS, 0.5% FCS, 0.5M EDTA) and fixed in PFA without undergoing permeabilization. Cells were gated on lymphocyte and monocyte populations and incubated with primary antibody (1/25) for 20 minutes at 4°C. The secondary antibody used was Phycoerythrin-conjugated rat polyclonal to mouse Ig, diluted 1/100. The PBMC cells were almost exclusively CD14+ monocytic cells.