製品の概要

  • 製品名
    Anti-Sialoadhesin antibody
    Sialoadhesin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sialoadhesin
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Sialoadhesin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Spleen Tissue Lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 183 kDa).

ターゲット情報

  • 機能
    Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
  • 組織特異性
    Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD169 antibody
    • CD169 antigen antibody
    • dJ1009E24.1 antibody
    • DKFZp667F058 antibody
    • FLJ00051 antibody
    • FLJ00055 antibody
    • FLJ00073 antibody
    • FLJ00411 antibody
    • FLJ32150 antibody
    • Sialic acid binding Ig like lectin 1 antibody
    • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
    • Sialic acid binding immunoglobulin like lectin 1 antibody
    • Sialic acid-binding Ig-like lectin 1 antibody
    • Sialoadhesin antibody
    • Siglec 1 antibody
    • Siglec-1 antibody
    • Siglec1 antibody
    • SN antibody
    • SN_HUMAN antibody
    see all

画像

  • Anti-Sialoadhesin antibody (ab94715) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 183 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,50 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

プロトコール

参考文献

ab94715 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Pig Cell lysate - other (Alveolar macrophages)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20000 cells
Specification
Alveolar macrophages
Blocking step
PBS/0.1% Tween 20/5% skimmed milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

David Winder

Verified customer

投稿 Aug 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録