Sialic Acid (NANA) Assay Kit (ab83375)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture media, Cell culture supernatant, Urine
- Sensitivity: 1 µM
製品の概要
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製品名
Sialic Acid (NANA) Assay Kit
Sialic acid キット 製品一覧 -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Urine, Cell culture media -
アッセイタイプ
Quantitative -
検出感度
1 µM -
検出範囲
0.1 µM - 10 µM -
全工程の試験時間
0h 40m -
製品の概要
Sialic Acid (NANA) Assay Kit (ab83375) is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.
The sialic acid assay uses an enzyme coupled reaction in which oxidation of free sialic acid creates an intermediate that reacts stoichiometrically with the probe to generate a product that can detected by absorbance (OD = 570 nm) or fluorescence (Ex/Em=535/587 nm).
The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 µM concentration.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K566 Sialic Acid (NANA) Colorimetric/Fluorometric Assay Kit. K566-100 is the same size as the 100 test size of ab83375.
Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer IV 1 x 25ml Development Enzyme Mix I 1 vial OxiRed Probe 1 x 200µl Sialic Acid Assay Buffer 1 x 25ml Sialic Acid Converting Enzyme 1 vial Sialic Acid Standard 1 vial -
研究分野
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関連性
Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been show recently that sialic acid level may associate with developmental and pathological stages. -
別名
- N acetylneuraminic acid
- NANA
画像
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Concentrations of free SA decreased (P < 0.0001), whereas bound and total SA increased (P < 0.0001) across lactation. Measured using ab83375.
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Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtrated
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Examples of colorimetric and fluorometric standard Sialic acid curves using ab83375.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab83375 は 9 報の論文で使用されています。
- Mealer RG et al. The schizophrenia-associated variant in SLC39A8 alters protein glycosylation in the mouse brain. Mol Psychiatry 27:1405-1415 (2022). PubMed: 35260802
- Dagenais M et al. Analysis of Schistosoma mansoni Extracellular Vesicles Surface Glycans Reveals Potential Immune Evasion Mechanism and New Insights on Their Origins of Biogenesis. Pathogens 10:N/A (2021). PubMed: 34832557
- Handigund M et al. N-acetylcysteine reduce the stress induced by cold storage of platelets: A potential way to extend shelf life of platelets. Transfus Apher Sci 60:103039 (2021). PubMed: 33388248
- Qaisar R et al. Circulating Biomarkers of Handgrip Strength and Lung Function in Chronic Obstructive Pulmonary Disease. Int J Chron Obstruct Pulmon Dis 15:311-321 (2020). PubMed: 32103930
- Marczynski M et al. Charged glycan residues critically contribute to the adsorption and lubricity of mucins. Colloids Surf B Biointerfaces 187:110614 (2020). PubMed: 31753616
- Gao Y et al. Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer. Cell Death Dis 9:416 (2018). PubMed: 29549328
- Chen WA et al. Addition of aGal HyperAcute™ technology to recombinant avian influenza vaccines induces strong low-dose antibody responses. PLoS One 12:e0182683 (2017). PubMed: 28787006
- Mudd AT et al. Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation. Front Nutr 3:39 (2016). Functional Studies . PubMed: 27660754
- Puerta-Guardo H et al. Dengue Virus NS1 Disrupts the Endothelial Glycocalyx, Leading to Hyperpermeability. PLoS Pathog 12:e1005738 (2016). PubMed: 27416066