製品の概要

  • 製品名Anti-Siah2 antibody
    Siah2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Siah2
  • アプリケーション適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human Siah2.

    (Peptide available as ab28592.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MCF7 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not. Promotes monoubiquitination of SNCA.
  • 組織特異性Widely expressed at low level.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the SINA (Seven in absentia) family.
    Contains 1 RING-type zinc finger.
    Contains 1 SIAH-type zinc finger.
  • ドメインThe RING-type zinc finger domain is essential for ubiquitin ligase activity.
    The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Partially nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase Siah2 antibody
    • hSiah2 antibody
    • Seven in absentia homolog 2 antibody
    • Siah E3 ubiquitin protein ligase 2 antibody
    • Siah-2 antibody
    • siah2 antibody
    • SIAH2_HUMAN antibody
    • Ubiquitin Ligase Siah2 antibody
    see all

Anti-Siah2 antibody 画像

  • Anti-Siah2 antibody (ab31234) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 49 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Siah2 antibody (ab31234) 使用論文

ab31234 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"