製品の概要

  • 製品名Anti-SIAH1 antibody
    SIAH1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SIAH1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: MSRQTATALP TGTSKCPPSQ RVPALTGTTA SNNDLASLFE CPVCFDYVLP, corresponding to N terminal amino acids 1-50 of Human SIAH1

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
  • 組織特異性Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the SINA (Seven in absentia) family.
    Contains 1 RING-type zinc finger.
    Contains 1 SIAH-type zinc finger.
  • ドメインThe RING-type zinc finger domain is essential for ubiquitin ligase activity.
    The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.
  • 翻訳後修飾Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase SIAH1 antibody
    • FLJ08065 antibody
    • hSIAH1 antibody
    • HUMSIAH antibody
    • Seven in absentia homolog 1 (Drosophila) antibody
    • Seven in absentia homolog 1 antibody
    • Siah 1 antibody
    • Siah 1a antibody
    • Siah E3 ubiquitin protein ligase 1 antibody
    • Siah-1 antibody
    • Siah-1a antibody
    • Siah1 antibody
    • SIAH1_HUMAN antibody
    • SIAH1A antibody
    • Ubiquitin ligase SIAH1 antibody
    see all

Anti-SIAH1 antibody 画像

  • All lanes : Anti-SIAH1 antibody (ab49146) at 0.5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 1 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

Anti-SIAH1 antibody (ab49146) 使用論文

ab49146 has not yet been referenced specifically in any publications.

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