製品の概要

  • 製品名Anti-SIAH1 antibody
    SIAH1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to SIAH1
  • アプリケーション適用あり: IHC-P, WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human SIAH1 aa 2-16 (N terminal).
    Sequence:

    SRQTATALPTGTSKC


    Database link: Q8IUQ4
    (Peptide available as ab22876)

  • ポジティブ・コントロール
    • Human liver lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 17189520

1:300 donkey anti-goat was used for secondary antibody.

WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28, 30 kDa (predicted molecular weight: 31, 35 kDa).Can be blocked with Human SIAH1 peptide (ab22876).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
  • 組織特異性Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the SINA (Seven in absentia) family.
    Contains 1 RING-type zinc finger.
    Contains 1 SIAH-type zinc finger.
  • ドメインThe RING-type zinc finger domain is essential for ubiquitin ligase activity.
    The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.
  • 翻訳後修飾Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase SIAH1 antibody
    • FLJ08065 antibody
    • hSIAH1 antibody
    • HUMSIAH antibody
    • Seven in absentia homolog 1 (Drosophila) antibody
    • Seven in absentia homolog 1 antibody
    • Siah 1 antibody
    • Siah 1a antibody
    • Siah E3 ubiquitin protein ligase 1 antibody
    • Siah-1 antibody
    • Siah-1a antibody
    • Siah1 antibody
    • SIAH1_HUMAN antibody
    • SIAH1A antibody
    • Ubiquitin ligase SIAH1 antibody
    see all

Anti-SIAH1 antibody 画像

  • All lanes : Anti-SIAH1 antibody (ab2237) at 1 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 31, 35 kDa
  • Anti-SIAH1 antibody (ab2237) at 1 µg/ml + Human liver lysate at 35 µg

    Predicted band size : 31, 35 kDa

Anti-SIAH1 antibody (ab2237) 使用論文

This product has been referenced in:
  • Snider NT  et al. Energy determinants GAPDH and NDPK act as genetic modifiers for hepatocyte inclusion formation. J Cell Biol 195:217-29 (2011). WB ; Mouse . Read more (PubMed: 22006949) »
  • Fujita K  et al. Positive feedback between p53 and TRF2 during telomere-damage signalling and cellular senescence. Nat Cell Biol 12:1205-12 (2010). WB ; Human . Read more (PubMed: 21057505) »

See all 5 Publications for this product

Product Wall

Thank you for calling Abcam earlier today.

I am sorry about the issues that you have been having with ab2237 in western blot. As we discussed on the phone, I am sending you a vial of ab49177 as a free of charge replacement. Your new order numb...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 10 µg
Specification Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

投稿 Oct 26 2011

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. This antibody is a relatively good selling antibody that we have received few complaints about. However, we do have one previous report of sim...

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Thank you for your enquiry. To date we have not received any customer reviews relating to the use of this product so I am unsure exactly what the non specific bands are that you are seeing. Have you utilised protease inhibitors and eliminated the p...

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I'm sorry to hear you are having a problem with ab2237 not detecting both bands. The two bands were detected in human brain lysate (the strongest was at 30kDa and faint at 45kDa) and it may be that the customer's cells express more of the 45kDa form th...

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The originator of ab2237 has confirmed for me that the antibody was characterized using human brain lysate (NOT U937 whole cell lysate). If you have any additional questions, please contact us again.

Thank you for your phone call. The datasheet for ab2287 is a bit confusing as it mentions both U937 lysate (U937 cells are a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an ...

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Two bands are detected with this antibody. The 30k band is much stronger and the size agrees with published reports. We don't know what the 45k band is.

No, Abcam does not source any of its products from Santa Cruz. This is a totally unique antibody that has recently been generated. If you do purchase it, please let us know how you get on and of course Abcam guarantees the product will work as it says ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"