製品の概要

  • 製品名Anti-SIAE antibody
  • 製品の詳細
    Mouse polyclonal to SIAE
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human SIAE protein (NP_733746.1)

  • ポジティブ・コントロール
    • Human colon tissue lysate. SIAE transfected 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

  • 関連性Sialic acids are acidic 9-carbon sugars typically found at the non-reducing end of sugar chains. They are frequently modified by 9-O-acetylation and this modification is removed by sialic acid acetylesterases. SIAE catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE appears to encode both lysosomal and cytosolic sialic acid acetylesterase isoforms (LSE and CSE, respectively). It is widely expressed with high expression in the testis, prostate, and colon. There are three named isoforms.
  • 細胞内局在Lysosome
  • 参照データベース
  • 別名
    • CSE C antibody
    • CSEC antibody
    • Cytosolic sialic acid 9 O acetylesterase homolog antibody
    • H Lse antibody
    • HLse antibody
    • LSE antibody
    • MGC87009 antibody
    • Sialate O acetylesterase antibody
    • Sialic acid acetylesterase antibody
    • Sialic acid specific 9 O acetylesterase antibody
    • Sialic acid specific acetylesterase II antibody
    • YSG2 antibody
    • Ysg2 homolog antibody
    see all

Anti-SIAE antibody 画像

  • Anti-SIAE antibody (ab69300) at 1/500 dilution + human colon tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 21 kDa,26 kDa,32 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SIAE antibody (ab69300) at 1/500 dilution

    Lane 1 : SIAE transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

Anti-SIAE antibody (ab69300) 使用論文

ab69300 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69300.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"